Impregnation (infiltration) is the process whereby the clearing agent is completely removed from the tissue and replaced by a medium that will completely fill all the tissue cavities thereby giving a firm consistency to the specimen, and allowing easier handling and cutting of suitably thin sections without any damage …19 Feb 2019

What is clearing infiltration impregnation and embedding?

IMPREGNATION OF THE TISSUES After Clearing, the tissues are infiltrated with a supporting medium which also replaces the clearing agent. … This process is also called as the internal embedding of the tissues as the embedding medium penetrates the tissues and provides the support from inside of the tissue.

What are the two functions of wax impregnation?

To remove bubbles from the tissue as may occur in porous tissues like lungs. To reduce the time of impregnation in case of dense tissue. To remove clearing agent more rapidly by increasing vaporization. In case of urgent biopsies.

Why must we clear before impregnation?

The medium needs to be eliminated during wax impregnation. This treatment is known as Clearing. Clearing agent also increases the refractive index of the tissues and thereby makes the tissues relatively more transparent.

What are types of embedding?

Embedding Media, Paraffin, Paramat, Paraplast, Peel Away Paraffin, Tissue Freezing Medium, Cryogenic-Gel, O.C.T. Compound, Polyfin, Polyester Wax.

What is impregnation method?

The impregnation method is simple and is the most popular catalyst preparation method. Usually a certain amount of support is immersed in a precursor solution. … The procedure involves the im- mersion of support powder into a precursor solution and filtration.

What is impregnation process?

Impregnation is the classic and the simplest procedure to load a given porous support with the metal component in the solid-state way (physical mixture of both component in solid state) or more commonly via wet impregnation (physical mixture of the support in solid state and the metal component dissolved in a liquid …

What is tissue impregnation?

Impregnation (infiltration) is the process whereby the clearing agent is completely removed from the tissue and replaced by a medium that will completely fill all the tissue cavities thereby giving a firm consistency to the specimen, and allowing easier handling and cutting of suitably thin sections without any damage …